Deriving energy from ATP hydrolysis, helicases alter the structure of DNA, RNA, or DNA:RNA duplexes, remodeling chromatin and modulating access to the DNA template by the transcriptional machinery. Does the helicase unzip? Helicase unzips the DNA strand. How does DNA unzip for transcription? DNA is copied into RNA in aRead More →
In the minimal in vitro transcription assay containing only these general transcription factors and purified RNA polymerase II, TBP remains bound to the TATA-box as the polymerase transcribes away from the promoter region, but the other general transcription factors dissociate. Does RNA polymerase bind to the operator? Between the promoterRead More →
Transcription takes place in the nucleus. It uses DNA as a template to make an RNA (mRNA) molecule. During transcription, a strand of mRNA is made that is complementary to a strand of DNA. Do both strands of DNA get transcribed? Unlike DNA replication, in which both strands are copied,Read More →
Transcription is the process by which the information in a strand of DNA is copied into a new molecule of messenger RNA (mRNA). What nucleic acid is Translation? Translation is catalyzed by a large enzyme called a ribosome, which contains proteins and ribosomal RNA (rRNA). Translation also involves specific RNARead More →
messenger RNA (mRNA), molecule in cells that carries codes from the DNA in the nucleus to the sites of protein synthesis in the cytoplasm (the ribosomes). The molecule that would eventually become known as mRNA was first described in 1956 by scientists Elliot Volkin and Lazarus Astrachan. How do theyRead More →
Go to Youtube and listen to conference speeches or presentations. … Start with short recordings and log your time. … When you’ve gotten used to one-speaker short recordings, start working with files that are longer with more speakers. How can I improve my transcription skills? Transcription Tips: 7 Ways toRead More →
Unlike DNA replication, in which both strands are copied, only one strand is transcribed. The strand that contains the gene is called the sense strand, while the complementary strand is the antisense strand. What is a single strand of DNA called? In both cases, replication occurs so quickly because multipleRead More →
The promoter will be a double stranded sequence at the end of the gene where RNA polymerase starts (= on 3′ end of template strand = on 5′ end of sense strand). Going along the sense strand, the way the gene is usually written (5′ to 3′, left to right)Read More →
The three post-transcriptional modifications are: 5′ capping, poly A tail addition, and splicing. What are post-transcriptional and post-translational process? Post-transcriptional mechanisms based on alternative splicing and RNA processing, as well as RNA silencing define the actual transcriptome supporting the cold stress response. Post-translational modifications such as ubiquitination and sumoylation regulateRead More →
Sets of transcription factor proteins bind to specific DNA sequences in or near a gene and promote or repress its transcription into an RNA. RNA processing. Splicing, capping, and addition of a poly-A tail to an RNA molecule can be regulated, and so can exit from the nucleus. What isRead More →