At ambient pressure, lysozyme is completely inactive against most gram-negative bacteria because it cannot penetrate the outer membrane to reach its target, the peptidoglycan. … However, pressure apparently stimulates passage of lysozyme through the outer membrane of several gram-negative bacteria. How do bacteria evade lysozyme? Pathogenic bacteria resist the bacteriolyticRead More →
Step 1: Resuspend a gram of cells in 1-2 ml buffer and incubate with 1mg/ml lysozyme with 0.5 mM EDTA in ice for 1 hour. Step 2: Freeze the cells using liquid nitrogen and that in water bath set at 37 degrees C. Step 3: Add some DNase and incubateRead More →
1) Lysozyme is a small enzyme that cuts sugar chains in bacterial cell walls, which causes bacteria to burst. It is used by our bodies to fight infections, and it is found naturally in egg whites, human tears, and mucus. What is the role of lysozyme in digestion? Lysozymes areRead More →