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In conclusion, isotype controls are useful for demonstrating that there was poor blocking of the cells. They should never be used to determine or set positivity in fully stained samples. How do you choose isotype control? How To Choose an Isotype Control. As a general rule of thumb, try toRead More →

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Isotype controls are used as negative controls to help differentiate non-specific background signal from specific antibody signal. Depending upon the isotype of the primary antibody used for detection and the target cell types involved, background signal may be a significant issue in various experiments. What is the purpose of isotypeRead More →